5NWX
Insight into the molecular recognition mechanism of the coactivator NCoA1 by STAT6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.7 |
Spacegroup name | P 62 |
Unit cell lengths | 61.631, 61.631, 73.283 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 53.370 - 2.510 |
R-factor | 0.18595 |
Rwork | 0.184 |
R-free | 0.23546 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oj5 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.948 |
Data reduction software | XDS |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.370 | 2.610 |
High resolution limit [Å] | 2.510 | 2.510 |
Rmeas | 0.025 | 0.132 |
Number of reflections | 5471 | |
<I/σ(I)> | 30.26 | |
Completeness [%] | 99.8 | 98.3 |
Redundancy | 20.51 | 19.92 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M sodium acetate, 0.1 M sodium cacodylate, pH 6.5, 30% PEG 8000, 5% PEG 400 |