5NW9
Crystal structure of the complex of Tdp1 with duplex DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 108.010, 195.310, 50.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.050 - 2.040 |
| R-factor | 0.2338 |
| Rwork | 0.232 |
| R-free | 0.27090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 1JY1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.261 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 52.050 |
| High resolution limit [Å] | 2.040 |
| Number of reflections | 69045 |
| <I/σ(I)> | 15.3 |
| Completeness [%] | 99.2 |
| Redundancy | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | 0.1M Ammonium sulphate, 0.1M HEPES, 25% w/v PEG 8000 |
