5NV8

Structural basis for EarP-mediated arginine glycosylation of translation elongation factor EF-P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.87290
Spacegroup name	I 4
Unit cell lengths	131.680, 131.680, 46.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.556 - 2.294
R-factor	0.292
Rwork	0.289
R-free	0.33940
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.664
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.542	2.360
High resolution limit [Å]	2.298	2.300
Rmerge	0.130	0.590
Rmeas	0.185	0.929
Number of reflections	17539	1760
<I/σ(I)>	7.73	1.87
Completeness [%]	97.1	98.49
Redundancy	3.5	3.3
CC(1/2)	0.993	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.2M ammonium acetate, 0.1M bis-tris and 27% (w/v) PEG 3350