5NV6
Structure of human transforming growth factor beta-induced protein (TGFBIp).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 61 |
Unit cell lengths | 114.840, 114.840, 181.240 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.930 |
R-factor | 0.22 |
Rwork | 0.219 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vxp |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.500 | 3.110 |
High resolution limit [Å] | 2.930 | 2.930 |
Rmerge | 0.165 | 1.112 |
Rmeas | 0.172 | 1.187 |
Number of reflections | 28689 | 4338 |
<I/σ(I)> | 17.1 | 1.9 |
Completeness [%] | 98.5 | 90.8 |
Redundancy | 13.1 | 8 |
CC(1/2) | 0.997 | 0.580 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 4% polyethylene glycol 4,000 0.1 M sodium acetate, pH 4.6 |