5NV1

SH3 domain from Mouse cortactin (C 2 2 21 crystal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.007997
Spacegroup name	C 2 2 21
Unit cell lengths	41.360, 55.950, 50.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.200 - 1.510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x69
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.200	1.580
High resolution limit [Å]	1.510	1.510
Rmerge	0.070	0.831
Number of reflections	9248
<I/σ(I)>	14.51
Completeness [%]	98.3	96.5
Redundancy	6	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	1.6 M Mg-sulfate, 0.1 M MES pH 6.5