5NUV

Structure of the WD40-domain of human ATG16L1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-30
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9184
Spacegroup name	C 1 2 1
Unit cell lengths	157.550, 38.220, 45.620
Unit cell angles	90.00, 105.74, 90.00

Refinement procedure

Resolution	43.909 - 1.550
R-factor	0.1666
Rwork	0.165
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cnx
RMSD bond length	0.009
RMSD bond angle	1.108
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.909	43.909	1.650
High resolution limit [Å]	1.550	10.000	1.550
Rmerge	0.067	0.034	0.730
Rmeas	0.073	0.037	0.792
Number of reflections	38280	160	6488
<I/σ(I)>	15.62	48.62	2.45
Completeness [%]	99.8	97.6	99.6
Redundancy	6.736	5.794	6.682
CC(1/2)	0.999	0.999	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.3	303	1.61 M (NH4)2SO4, 0.09 M NaCl, 0.1 M MES pH 6.3