5NTU

Crystal Structure of human Pro-myostatin Precursor at 2.6 A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	C 1 2 1
Unit cell lengths	168.157, 36.301, 120.448
Unit cell angles	90.00, 104.39, 90.00

Refinement procedure

Resolution	28.270 - 2.580
R-factor	0.217
Rwork	0.215
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.550
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	76.264	76.264	2.620
High resolution limit [Å]	2.575	6.989	2.575
Rmeas	0.071	0.046	1.037
Rpim	0.034	0.022	0.493
Total number of observations	90386
Number of reflections	22474
<I/σ(I)>	11.7
Completeness [%]	97.7	99.1	99.6
Redundancy	4	3.6	4.2
CC(1/2)	0.997	0.997	0.583

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	292	10% PEG6000, 0.1M HEPES: cryo-protection: 70% ethylene glycol