5NTG
Structure of Leucyl aminopeptidase from Trypanosoma cruzi in complex with citrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 160.120, 160.120, 203.672 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.310 - 2.320 |
| R-factor | 0.16772 |
| Rwork | 0.166 |
| R-free | 0.20997 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nsk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.544 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.310 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.188 | 1.202 |
| Number of reflections | 43546 | 4249 |
| <I/σ(I)> | 11 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Phosphate-citrate pH 4.2, 40 % (v/v) PEG 300, 5 mM MnSO4, 5 mM amastatin |
