5NT1

Complex of influenza A NS1 effector domain with TRIM25 coiled coil

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	C 2 2 2
Unit cell lengths	131.380, 225.230, 146.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.685 - 2.820
R-factor	0.1964
Rwork	0.195
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ltb
RMSD bond length	0.006
RMSD bond angle	0.800
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.680	2.890
High resolution limit [Å]	2.820	2.820
Rmerge	0.092
Rpim	0.042
Number of reflections	52603	3878
<I/σ(I)>	14.9	1.4
Completeness [%]	99.9	100
Redundancy	6.8
CC(1/2)	1.000	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	100 mM Tris pH 8.5 25 % PEG 600 200 mM sodium citrate