5NST
Human monoclonal antibody with a LAIR1 insertion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.823, 86.462, 103.998 |
| Unit cell angles | 90.00, 126.65, 90.00 |
Refinement procedure
| Resolution | 81.900 - 2.520 |
| R-factor | 0.2214 |
| Rwork | 0.219 |
| R-free | 0.26730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3KGR and 3DIF |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 81.900 |
| High resolution limit [Å] | 2.520 |
| Number of reflections | 40863 |
| <I/σ(I)> | 7.4 |
| Completeness [%] | 99.8 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% PEG 4000, 0.1M sodium citrate pH 4.5 |
