5NSL
Structure of D80A-fructofuranosidase from Xanthophyllomyces dendrorhous complexed with fructose and hydroxytyrosol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.860, 206.124, 145.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 118.880 - 1.700 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.18900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5ann |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.344 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.110 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.058 | |
| Number of reflections | 247009 | |
| <I/σ(I)> | 18.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | Protein was crystallized from 1.3M Sodium Citrate tribasic dihydrate |
