5NSK
apo Structure of Leucyl aminopeptidase from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 162.330, 162.450, 176.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.610 - 2.600 |
| R-factor | 0.19212 |
| Rwork | 0.191 |
| R-free | 0.21085 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2HB6 poly-Ala model |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.532 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.610 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.165 | 1.019 |
| Number of reflections | 143992 | |
| <I/σ(I)> | 9.5 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.8 M Na citrate pH 7.0, 5 mM CoSO4 |
