5NRO
Structure of full-length DnaK with bound J-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-30 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.872899 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 127.246, 127.246, 252.885 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.121 - 3.250 |
| R-factor | 0.2168 |
| Rwork | 0.216 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b9q |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.647 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.121 | 3.510 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rpim | 0.056 | 0.608 |
| Number of reflections | 19859 | 3983 |
| <I/σ(I)> | 13.8 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.4 | 20.1 |
| CC(1/2) | 0.999 | 0.515 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 9% w/v PEG 8000 0.1M calcium acetate 0.1 M imidazole pH 6.5 protein at 18 mg/ml crystalization drop contained 200 nl protein + 100 nl reservoir solution |
