5NR1
FzlA from C. crescentus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-06-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54184 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 124.326, 124.326, 124.326 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.800 - 2.004 |
| R-factor | 0.1631 |
| Rwork | 0.161 |
| R-free | 0.20010 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.298 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1036) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.087 | 0.448 |
| Rpim | 0.043 | 0.226 |
| Number of reflections | 40294 | 2792 |
| <I/σ(I)> | 12.7 | |
| Completeness [%] | 97.3 | 92.6 |
| Redundancy | 4.9 | 4.7 |
| CC(1/2) | 0.997 | 0.857 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 50% PEG 200, 0.2 M NaCl, 0.1 M sodium potassium phosphate pH 6.2 |
