5NQV

Structure of the Arabidopsis Thaliana TOPLESS N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 43 21 2
Unit cell lengths	94.100, 94.100, 298.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.550 - 1.950
R-factor	0.191
Rwork	0.190
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nqs
RMSD bond length	0.010
RMSD bond angle	0.930
Data reduction software	XDS (VERSION November 3, 2014)
Data scaling software	XSCALE (VERSION November 3, 2014)
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.070
High resolution limit [Å]	1.950	1.950
Number of reflections	709778	115219
<I/σ(I)>	13.12	1.2
Completeness [%]	98.1	97.2
Redundancy	7.33	7.57
CC(1/2)	0.999	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Di-Ammonium tartrate 1.08 M pH 7 and 2% benzamidine-HCl