5NQS

Structure of the Arabidopsis Thaliana TOPLESS N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-21
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.873
Spacegroup name	P 31 1 2
Unit cell lengths	72.681, 72.681, 181.147
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.910 - 2.610
R-factor	0.2027
Rwork	0.200
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	partially built MAD model
RMSD bond length	0.010
RMSD bond angle	1.090
Data reduction software	XDS ((VERSION January 10, 2014)
Data scaling software	XSCALE ((VERSION January 10, 2014)
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.700
High resolution limit [Å]	2.610	2.610
Number of reflections	16949	10538
<I/σ(I)>	12.2	1.79
Completeness [%]	99.7	100
Redundancy	6.8	6.48
CC(1/2)		0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Na2HPO4-Citrate 0.2 M pH 4.2, 2-propanol 10% v/v and Lithium sulfate 0.3 M