5NQ9
Crystal structure of laccases from Pycnoporus sanguineus, izoform II, monoclinic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.459, 61.962, 103.307 |
| Unit cell angles | 90.00, 109.82, 90.00 |
Refinement procedure
| Resolution | 48.650 - 2.720 |
| R-factor | 0.1737 |
| Rwork | 0.170 |
| R-free | 0.24649 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xyb |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.857 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.880 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmerge | 0.128 | 0.766 |
| Number of reflections | 29229 | |
| <I/σ(I)> | 10.38 | 1.85 |
| Completeness [%] | 99.3 | 97.5 |
| Redundancy | 3.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M magnesium formate dihydrate 20% PEG 3350 |
