5NQ7

Crystal structure of laccases from Pycnoporus sanguineus, izoform I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	P 43 3 2
Unit cell lengths	185.550, 185.550, 185.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.390 - 2.750
R-factor	0.16367
Rwork	0.161
R-free	0.20736
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xyb
RMSD bond length	0.012
RMSD bond angle	1.604
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.750	2.750
Rmerge	0.129	1.120
Number of reflections	28947	2870
<I/σ(I)>	27.7	3.78
Completeness [%]	100.0	100
Redundancy	25.8	26.8
CC(1/2)	1.000	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M HEPES pH 7.5 2.0 M ammonium sulfate