5NPT

Structure of the N-terminal domain of the yeast telomerase reverse transcriptase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97858
Spacegroup name	I 21 21 21
Unit cell lengths	38.195, 64.098, 126.064
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.030 - 2.400
R-factor	0.18806
Rwork	0.186
R-free	0.22364
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	INCOMPLETE MODEL OF SE-MET VARIANT SOLVED BY SAD METHOD USING CRANK2 SOFTWARE
RMSD bond length	0.015
RMSD bond angle	1.690
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.030	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.047	0.347
Rpim	0.029	0.194
Number of reflections	6202	3177
<I/σ(I)>	20.6
Completeness [%]	98.0	98.9
Redundancy	5	4.7
CC(1/2)	0.998	0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	10.5	293	Sodium-potassium-phosphate, sodium citrate and CAPS buffer