5NP9
Crystal structure of Bacillus subtilis YdiB in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976250 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.030, 111.770, 35.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.059 - 2.000 |
| R-factor | 0.1768 |
| Rwork | 0.174 |
| R-free | 0.22780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mvr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.934 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.060 |
| High resolution limit [Å] | 2.050 |
| Number of reflections | 10574 |
| <I/σ(I)> | 8.2 |
| Completeness [%] | 97.1 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 30% PEG 4000, 200 mM MgCl2 and 100 mM Tris pH 8.5 |
