5NNP
Structure of Naa15/Naa10 bound to HypK-THB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-01 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.872900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.319, 106.041, 130.937 |
| Unit cell angles | 90.00, 94.98, 90.00 |
Refinement procedure
| Resolution | 49.321 - 2.602 |
| R-factor | 0.164 |
| Rwork | 0.161 |
| R-free | 0.21990 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.967 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.321 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rpim | 0.100 | |
| Number of reflections | 77910 | 7407 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 99.7 | 97.3 |
| Redundancy | 6.9 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Tris pH 8.5 25 % PEG 3350 protein at 15 mg/ml concentration |
