5NN7
KSHV uracil-DNA glycosylase, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.873, 43.038, 57.387 |
| Unit cell angles | 90.00, 99.34, 90.00 |
Refinement procedure
| Resolution | 56.630 - 2.500 |
| R-factor | 0.2074 |
| Rwork | 0.206 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j8x |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.081 |
| Data reduction software | DIALS (1.3) |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.630 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.061 | 0.134 |
| Rpim | 0.051 | 0.125 |
| Number of reflections | 7316 | |
| <I/σ(I)> | 13.1 | 6.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 3.3 | 3.4 |
| CC(1/2) | 0.996 | 0.978 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1 M bis-tris, 0.2 M sodium chloride, 25% (w/v) PEG 3350 |
