5NMZ
human Neurturin (97-197)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.433, 50.794, 53.642 |
| Unit cell angles | 76.42, 89.33, 94.74 |
Refinement procedure
| Resolution | 52.120 - 1.600 |
| R-factor | 0.232 |
| Rwork | 0.231 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mr9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.120 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 37888 | |
| <I/σ(I)> | 9.78 | 1.93 |
| Completeness [%] | 89.8 | 90.1 |
| Redundancy | 1.8 | 1.8 |
| CC(1/2) | 0.998 | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 3 M Na formate, 0.1 M Bis Tris pH 6 |
