5NLG
RRP5 C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 114.010, 114.010, 65.740 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.476 - 2.350 |
| R-factor | 0.2019 |
| Rwork | 0.198 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.890 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.476 | 39.476 | 2.410 |
| High resolution limit [Å] | 2.350 | 10.510 | 2.350 |
| Rmerge | 0.093 | 0.025 | 0.973 |
| Rmeas | 0.104 | 0.029 | 1.102 |
| Total number of observations | 106545 | ||
| Number of reflections | 20756 | 269 | 1491 |
| <I/σ(I)> | 13.17 | 45.94 | 1.72 |
| Completeness [%] | 99.8 | 97.8 | 98.6 |
| Redundancy | 5.133 | 4.621 | 4.446 |
| CC(1/2) | 0.998 | 0.999 | 0.664 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 16-21% PEG1000 100mM Sodium/potassium phosphate, pH 6.0 |
