5NLF
Crystal structure of Zn2.7-E16V human ubiquitin (hUb) mutant adduct, from a solution 100 mM zinc acetate/1.3 mM E16V hUb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-03-16 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.265 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.410, 50.530, 93.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.840 - 1.500 |
| R-factor | 0.15736 |
| Rwork | 0.156 |
| R-free | 0.18033 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ehv |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.433 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.840 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 32385 | 4199 |
| <I/σ(I)> | 18.2 | 6.2 |
| Completeness [%] | 94.2 | 85.2 |
| Redundancy | 10 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22-30% (w/v) PEG 1450, 50 mM HEPES, 100 mM Zn(CH3COO)2 |
