5NK0

Crystal Structure of Ephrin A2 (EphA2) Receptor Protein Kinase with Compound 1j

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-07
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.97795
Spacegroup name	P 1 21 1
Unit cell lengths	32.318, 104.838, 39.259
Unit cell angles	90.00, 94.53, 90.00

Refinement procedure

Resolution	39.136 - 1.597
R-factor	0.165
Rwork	0.164
R-free	0.19380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i9u
RMSD bond length	0.007
RMSD bond angle	1.075
Data reduction software	XDS (XDSapp 2.0)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.136	1.690
High resolution limit [Å]	1.597	1.600
Rmeas	0.099	1.102
Number of reflections	33701
<I/σ(I)>	14.29	1.82
Completeness [%]	97.7	95.1
Redundancy	6.95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	37.5% MPD/PEG1000/PEG3350 (MD), 0.175 M Amino Acids Mix (MD), 0.1 M Buffer System 3 (MD) pH8.5