5NIY
Signal recognition particle-docking protein FtsY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.976251 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.730, 80.640, 59.307 |
| Unit cell angles | 90.00, 95.23, 90.00 |
Refinement procedure
| Resolution | 47.648 - 1.700 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.19470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yhs |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.813 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.466 | |
| Number of reflections | 33675 | |
| <I/σ(I)> | 14.2 | 3 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Ammonium acetate, 0.1 M BIS-TRIS pH 5.5, 17% w/v Polyethylene glycol 10,000 |
