5NIV
Crystal structure of 5D3 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.203, 45.193, 83.278 |
| Unit cell angles | 90.00, 122.14, 90.00 |
Refinement procedure
| Resolution | 42.197 - 1.498 |
| R-factor | 0.1819 |
| Rwork | 0.181 |
| R-free | 0.20530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cad |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.995 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.197 | 1.550 |
| High resolution limit [Å] | 1.498 | 1.500 |
| Rmerge | 0.073 | 0.566 |
| Number of reflections | 66575 | |
| <I/σ(I)> | 16.9 | 4.2 |
| Completeness [%] | 94.1 | 88.2 |
| Redundancy | 7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris pH 8.5 and 16% PEG 6000 |
