5NIT
Glucose oxidase mutant A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-05-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 128.657, 128.657, 77.693 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 111.420 - 1.870 |
| R-factor | 0.16678 |
| Rwork | 0.165 |
| R-free | 0.20393 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cf3 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.952 |
| Data reduction software | iMOSFLM (7.2.1) |
| Data scaling software | SCALA (6.5.019) |
| Phasing software | PHASER (7.0.20) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.270 |
| High resolution limit [Å] | 1.860 |
| Number of reflections | 61296 |
| <I/σ(I)> | 12.6 |
| Completeness [%] | 98.1 |
| Redundancy | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 280 | 100 mM HEPES 40% v/v dioxane |
