5NIO
EthR complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9174 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 120.083, 120.083, 33.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 84.910 - 1.400 |
| R-factor | 0.18214 |
| Rwork | 0.181 |
| R-free | 0.20498 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.682 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.910 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.034 | 0.400 |
| Number of reflections | 48919 | 16586 |
| <I/σ(I)> | 22.6 | |
| Completeness [%] | 99.2 | 96.2 |
| Redundancy | 5.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | MES buffer, 1.4-1.6 M AS |
