5NIJ
Crystal structure of arabidopsis thaliana legumain isoform gamma in two-chain activation state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-30 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 41 |
| Unit cell lengths | 147.830, 147.830, 101.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.112 - 2.750 |
| R-factor | 0.2155 |
| Rwork | 0.214 |
| R-free | 0.25230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fgu |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.714 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.860 | 2.830 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.098 | 0.770 |
| Number of reflections | 56996 | |
| <I/σ(I)> | 8.6 | 1.6 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES pH 6.5 and 1.6 M MgSO4 |
