5NI3
sfGFP 204-204 mutant dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-07 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.700, 98.020, 102.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.170 - 1.280 |
| R-factor | 0.17495 |
| Rwork | 0.174 |
| R-free | 0.19102 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bt0 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.264 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 57.170 |
| High resolution limit [Å] | 1.280 |
| Number of reflections | 249821 |
| <I/σ(I)> | 17.7 |
| Completeness [%] | 99.9 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | PACT condition C6: 0.1M PCTP Buffer, pH 9.0, 25% PEG 1500 |
