5NHW

CRYSTAL STRUCTURE OF THE BIMAGRUMAB Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.99990
Spacegroup name	P 21 21 21
Unit cell lengths	44.021, 78.016, 131.288
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.740 - 1.780
R-factor	0.1794
Rwork	0.178
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jb5
RMSD bond length	0.010
RMSD bond angle	1.080
Data reduction software	HKL
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	1.840
High resolution limit [Å]	1.780	3.830	1.780
Rmerge	0.094	0.047	0.470
Total number of observations	276646
Number of reflections	44353
<I/σ(I)>	6
Completeness [%]	100.0	99.9	100
Redundancy	6.2	6.8	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	18% PEG 5000 MME, 50mM TRIS