5NFY
SARS-CoV nsp10/nsp14 dynamic complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2012-04-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.28348 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 185.881, 189.786, 196.245 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.398 - 3.382 |
| R-factor | 0.1947 |
| Rwork | 0.193 |
| R-free | 0.23490 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.978 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.893 | 3.393 |
| High resolution limit [Å] | 3.382 | 3.382 |
| Rmerge | 0.129 | 1.139 |
| Rpim | 0.046 | 0.395 |
| Number of reflections | 97097 | 968 |
| <I/σ(I)> | 15.1 | 2 |
| Completeness [%] | 99.6 | 98.6 |
| Redundancy | 9 | 9.3 |
| CC(1/2) | 0.988 | 0.681 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277.15 | 0.1M Sodium citrate tribasic pH5.5; 8%PEG 8000; 30% Hexandiol |
