5NFG
Structure of recombinant cardosin B from Cynara cardunculus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.580, 143.130, 64.710 |
| Unit cell angles | 90.00, 107.90, 90.00 |
Refinement procedure
| Resolution | 61.578 - 2.375 |
| R-factor | 0.2239 |
| Rwork | 0.222 |
| R-free | 0.26970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b5f |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.110 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 61.600 |
| High resolution limit [Å] | 2.375 |
| Number of reflections | 36375 |
| <I/σ(I)> | 7.3 |
| Completeness [%] | 99.2 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 150mM KBr, 30% PEG 2000 MME |
