5NEF
The structure of the G. violaceus guanidine II riboswitch P1 stem-loop with guanidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 55.700, 55.700, 132.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.187 - 1.910 |
| R-factor | 0.2054 |
| Rwork | 0.203 |
| R-free | 0.25790 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.877 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2219: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.190 | 1.940 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.085 | 1.500 |
| Rpim | 0.030 | 0.725 |
| Number of reflections | 11824 | 313 |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 9.4 | 9.6 |
| CC(1/2) | 0.999 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 280 | 0.15 M KCl 25% v/v Glycerol 0.05M Bis-Tris 7.0 1.6M Ammonium sulfate |
