5NDG

Crystal structure of geneticin (G418) bound to the yeast 80S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	90
Detector technology	PIXEL
Collection date	2014-11-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	443.590, 297.320, 300.150
Unit cell angles	90.00, 99.28, 90.00

Refinement procedure

Resolution	91.755 - 3.700
R-factor	0.2105
Rwork	0.210
R-free	0.25300
RMSD bond length	0.009
RMSD bond angle	1.447
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	91.755	115.000	3.800
High resolution limit [Å]	3.700	100.000	3.700
Rmerge	0.159	12.300	1.697
Rmeas	0.176	17.395	1.892
Number of reflections	814796	1	62491
<I/σ(I)>	8.28		0.9
Completeness [%]	99.9	7.1	99.9
Redundancy	5.209	2	5.034
CC(1/2)	0.998		0.478

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine