5NCM
Crystal structure Cbk1(NTR)-Mob2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 126.270, 126.270, 49.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.930 - 2.800 |
| R-factor | 0.2508 |
| Rwork | 0.249 |
| R-free | 0.28380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5brk |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.696 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2420: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.930 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 1.520 |
| Number of reflections | 10323 | 750 |
| <I/σ(I)> | 21.8 | 2.21 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 14 | 14.7 |
| CC(1/2) | 1.000 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 100mM MES/Imidazole, 30mM CaCl 2 , 30mM MgCl 2 , 12.5% (w/v) PEG1000, 12.5% (w/v) PEG3350, 12.5% (v/v) MPD |
