5NBH
Structure of the distal domain of mouse adenovirus 2 fibre, P212121 native
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.872, 91.110, 128.922 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.050 - 1.700 |
| R-factor | 0.17422 |
| Rwork | 0.172 |
| R-free | 0.21621 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n8d |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.509 |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.100 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.116 | 0.233 |
| Number of reflections | 68912 | 3722 |
| <I/σ(I)> | 6.3 | 2.1 |
| Completeness [%] | 94.3 | 69.6 |
| Redundancy | 3.5 | 1.9 |
| CC(1/2) | 0.983 | 0.857 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 10 mM Tris-HCl, 10 % (w/v) PEG 4000, 0.1 M HEPES-NaOH, 0.1 M magnesium chloride |
