5NB7
Complement factor D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.801, 49.884, 39.088 |
| Unit cell angles | 90.00, 106.08, 90.00 |
Refinement procedure
| Resolution | 29.070 - 1.330 |
| R-factor | 0.14299 |
| Rwork | 0.140 |
| R-free | 0.19582 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dsu |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.560 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.390 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Number of reflections | 47029 | 5778 |
| <I/σ(I)> | 18.51 | 3.6 |
| Completeness [%] | 99.0 | 98.6 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 22% PEG 3350, 0.1 m HEPES pH 7.5, 50 mM NaCl |
