5NAQ

Crystal structure of native 6-phospho-glucosidase LpBgl from Lactobacillus plantarum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM16
Synchrotron site	ESRF
Beamline	BM16
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-28
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	196.688, 191.683, 105.931
Unit cell angles	90.00, 102.75, 90.00

Refinement procedure

Resolution	61.233 - 2.480
R-factor	0.1618
Rwork	0.159
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cmj
RMSD bond length	0.008
RMSD bond angle	0.993
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.233	2.610
High resolution limit [Å]	2.480	2.480
Rmerge	0.117	0.431
Rpim	0.076	0.281
Number of reflections	126809	18978
<I/σ(I)>	6.9	2.3
Completeness [%]	94.0	96.5
Redundancy	3	2.9
CC(1/2)	0.987	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	15% (w/v) PEG 3350, 0.2 M di-ammonium phosphate, 0.1 M sodium cacodylate, pH 6.4, 2 mM DTT and detergent CTAB.