5NAA
Lipoprotein-releasing system transmembrane protein LolC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 54.680, 111.780, 75.939 |
Unit cell angles | 90.00, 108.97, 90.00 |
Refinement procedure
Resolution | 45.990 - 1.880 |
R-factor | 0.18223 |
Rwork | 0.180 |
R-free | 0.22520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lil |
RMSD bond length | 0.017 |
RMSD bond angle | 1.806 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.990 | 1.920 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.078 | 0.436 |
Number of reflections | 34822 | |
<I/σ(I)> | 11 | |
Completeness [%] | 99.5 | |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 288.15 | 0.2 M Ammonium sulfate, 0.1 M Sodium acetate pH 4.6, 30% w/v PEG 2000 MME |