5N9O

EGFP(enhanced green fluorescent protein) mutant - L232H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.972
Spacegroup name	C 1 2 1
Unit cell lengths	84.595, 67.931, 47.266
Unit cell angles	90.00, 118.66, 90.00

Refinement procedure

Resolution	12.500 - 1.530
R-factor	0.16948
Rwork	0.167
R-free	0.21146
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4l12
RMSD bond length	0.021
RMSD bond angle	2.509
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.480	1.580
High resolution limit [Å]	1.530	1.530
Rmerge	0.073	0.510
Rpim	0.048	0.380
Number of reflections	34978	3431
<I/σ(I)>	8.6	1.8
Completeness [%]	98.8	99.5
Redundancy	3.3	3.3
CC(1/2)		0.589

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	288	2-propanol , MES monohydrate, PEG 2000