5N9B

Crystal Structure of unliganded human IL-17RA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.000010
Spacegroup name	P 21 21 21
Unit cell lengths	40.593, 71.685, 105.385
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.270 - 1.900
R-factor	0.192
Rwork	0.190
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hsa
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.270	59.270	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.104	0.042	1.125
Rmeas	0.115	0.048	1.223
Total number of observations	156084
Number of reflections	24921	326	1807
<I/σ(I)>	11.8	35.11	1.73
Completeness [%]	99.7	97	99.9
Redundancy	6.263	5.129	6.531
CC(1/2)	0.997	0.998	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	Tris-HCl, PEG 2000 MME, lithium sulfate