5N99

CRYSTAL STRUCTURE OF STREPTAVIDIN with cyclic peptide NQpWQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.99999
Spacegroup name	P 21 21 21
Unit cell lengths	67.744, 115.315, 210.369
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.720 - 1.500
R-factor	0.1684
Rwork	0.167
R-free	0.19170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.720	1.600
High resolution limit [Å]	1.500	1.500
Number of reflections	261661	44516
<I/σ(I)>	13.3	1.49
Completeness [%]	99.7	98.4
Redundancy	6.58	5.77
CC(1/2)	0.999	0.447

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1M Sodium citrate pH 5.5 15% PEG6000