5N8T
CRYSTAL STRUCTURE OF STREPTAVIDIN D-amino acid containing peptide Gdlwqheatwkkq
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.700001 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 57.779, 57.779, 84.405 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.720 - 1.610 |
R-factor | 0.20587 |
Rwork | 0.204 |
R-free | 0.24341 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.526 |
Data reduction software | XDS |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.720 | 1.710 |
High resolution limit [Å] | 1.610 | 1.610 |
Rmerge | 0.950 | |
Number of reflections | 18228 | 3098 |
<I/σ(I)> | 14.94 | 0.86 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.48 | |
CC(1/2) | 0.990 | 0.341 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1M Sodium citrate pH 4.5, 20% PEG4000 |