5N7D

MAGI-1 complexed with a RSK1 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	195.810, 60.680, 99.710
Unit cell angles	90.00, 98.81, 90.00

Refinement procedure

Resolution	49.267 - 2.300
R-factor	0.1923
Rwork	0.191
R-free	0.22710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xjl
RMSD bond length	0.003
RMSD bond angle	0.569
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_2420: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.267	2.360
High resolution limit [Å]	2.300	2.300
Rmeas		0.773
Number of reflections	51873
<I/σ(I)>	18	2.36
Completeness [%]	100.0	100
Redundancy	12.8	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	296	8% PEG 400, 100 mM acetate buffer