5N78
Crystal structure of the cytosolic domain of the CorA Mg2+ channel from Escherichia coli in complex with magnesium and cobalt hexammine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979, 1.6049 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.721, 117.316, 91.858 |
| Unit cell angles | 90.00, 103.14, 90.00 |
Refinement procedure
| Resolution | 29.440 - 2.850 |
| R-factor | 0.17969 |
| Rwork | 0.178 |
| R-free | 0.21772 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.431 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.450 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.121 | 0.910 |
| Number of reflections | 35009 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 98.2 | 89.4 |
| Redundancy | 5.7 | |
| CC(1/2) | 0.997 | 0.671 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 34 mM CoHex, 20 mM MgCl2, 100 mM Glycine pH 8.7, 30% PEG400, 100 mM NaCl |
