5N6P
AMPA receptor NTD mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 94.500, 94.500, 160.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.710 - 2.800 |
| R-factor | 0.20817 |
| Rwork | 0.206 |
| R-free | 0.24933 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hsy |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.750 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.710 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.119 | 0.846 |
| Rpim | 0.069 | 0.483 |
| Number of reflections | 18494 | 2683 |
| <I/σ(I)> | 9.4 | 1.9 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.9 | 4 |
| CC(1/2) | 0.993 | 0.498 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 16-20% PEG3350, 200 mM sodium formate |
