5N6F
Crystal structure of TGT in complex with guanine fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.711, 64.851, 70.655 |
| Unit cell angles | 90.00, 93.36, 90.00 |
Refinement procedure
| Resolution | 44.279 - 1.119 |
| R-factor | 0.136782737006 |
| Rwork | 0.136 |
| R-free | 0.15350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lbu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.962 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.280 | 1.190 |
| High resolution limit [Å] | 1.119 | 1.119 |
| Rmerge | 0.487 | |
| Number of reflections | 149008 | 149008 |
| <I/σ(I)> | 10.3 | 1.96 |
| Completeness [%] | 97.1 | 83.2 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 13% PEG8000, 100MM MES, 1MM DTT, 10% DMSO |
